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The authors noted that human research remains limited to small pilot studies, and that no major adverse effects have been reported in published animal or early human work

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Digestion was performed using 23 mL of collagenase media [prepared with 4 mL Dispase (Corning, 354235), 40 L of 0.05 mg/mL DNase I (Sigma, DN25), 4 L anti-mouse CD16/32 antibody (Biolegend, 101302), 200 L collagenase I (Gibco, 17100017), and 10 M Y-27632 (APExBIO, A3008)]

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Though we still have much to learn about the factors that contribute to autism, research suggests that a combination of genetic and environmental factors have a role